Two orange reference bands at 70 kda and 25 kda and one green reference band at 10 kda highlight the bluestained protein ladder. The hxstable prestained protein ladder is a threecolor protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kda. Hxstable prestained protein ladder dna protein ladders. Prestained protein ladders and unstained protein ladders abcam. Pageruler prestained protein ladder 10 170 kda migration patterns were determined using respective nupage precast gels.
Home life science research biorad products electrophoresis and blotting protein electrophoresis and western blotting protein standards and ladders molecular weight markers recombinant protein standards. This reagent must be used in combination with cruz marker mw tag detection antibodies available only from santa cruz biotechnology, inc. The blueray prestained protein marker is a threecolor protein standard with 10 prestained purified proteins. Covalently coupled dyes allows easy visualization of the ladder during, and after electrophoresis. Mxstable prestained protein ladder is a threecolor protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180 kda. The thermo scientific pageruler plus prestained protein ladders are a mixture of nine recombinant proteins ranging from 10 kda to 250 kda. The color prestained protein standard, broad range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 72 kda and 26 kda, respectively, that resolves into 11 sharp bands when electrophoresed. Pink plus prestained protein ladder 10175kda cleaver. Load the following volumes of the ladder on sdspage gel. Thermo scientific pageruler prestained protein ladders the thermo scientific pageruler and pageruler plus prestained protein ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination.
Datasheet for colorplus prestained protein ladder, broad. The pack contains biotinylated protein ladder and antibiotin, hrplinked antibody. Pageruler prestained protein ladder is a mixture of ten blue, orange, and greenstained recombinant proteins 10 to 170 kda for use as size standards in protein electrophoresis sdspage and western blotting. Prestained protein ladders and unstained protein ladders. Each marker is covalently bound to a pink or blue colour chromaphore to produce a ladder of evenly. So, does pierce pageruler prestained protein ladder a mix of phosphorylated protein. Download a pdf containing pricing for our full product list. Color prestained protein standard, broad range 11245 kda neb. Benchmark prestained protein ladder contains 10 proteins ranging in apparent molecular weight from 6 to 180 kda for use in monitoring electrophoresis or. Thermo scientific pierce protein molecular weight marker. It is prestained and has multicoloured peptides which makes it easy to identify the band of the protein of interest. Biovisions briteruler prestained protein ladder is a readytouse formulation which does not require boiling, contains 10 prestained proteins covering a wide range of molecular weights 10180 kda and includes a red reference band at 75 kda and a green reference band at 25 kda. Blueelf prestained protein marker, prestained protein mw marker.
Thermo scientific pageruler plus prestained protein ladder, 10 to 250 kda mixture of 9 blue, orange and greenstained proteins 10 to 250kda used as size standards in protein electrophoresis sdspage and western blotting. Prestained protein marker triple color, edta free, 10250. Three different chromophores are bound to the proteins, 9 bands with blue, one red at 70 kda and one green at 25 kda. The ladder is visualized by sdspage using coomassie or silver stains or detected in western blots with protein stains. With, one of our best sellers, 3 tricolored prestained protein marker, you can load up over 300 wells for western blotting application.
Prestained protein marker, broad range 7175 kda neb. Get to know more about ketogenic diet and pageruler prestained protein ladder concentration here on this site. However, an unstained protein ladder can only be visualized following staining with coomassie, or a similar nonspecific protein stain. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions. Proteins are covalently coupled with a blue chromophore except for two reference bands one. Blue prestained protein standard, broad range 11250 kda. Proteins are covalently coupled with a blue chromophore except for two reference bands one green.
Test our acuteband prestained protein ladder mw range 6. The pink plus prestained protein ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. Fig 7 pageruler prestained protein ladder thermo scientific ltd page 12 iii from bioc 46 at the university of hong kong. Bluerayprestainedproteinmarker 10180kda readytouseprestainedproteinladder cat. Color prestained protein standard, broad range is a mixture of highly purified, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 80 kda and 25 kda, respectively, that resolves into 12 sharp bands when electrophoresed. Resolving and identifying protein of interest using. The apparent molecular weights of the colorplus prestained. Proteins are covalently coupled with a blue chromophore except for two reference bands one green and one red band when. Thermo scientific pageruler unstained broad range protein. The readytouse bluestain protein ladder covers a molecular weight between 24510 kda, and is used for protein electrophoresis operations. Prestained protien ladder molecular biology proteomics.
Broad range prestained protein marker3245 kda pl00002. For easy reference, the 50 kda protein band has a greater intensity than the other proteins in the ladder. The accuruler prestained protein ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kda. Description this prestained protein mw marker is designed for monitoring the progress of sdspolyacrylamide gel electrophoresis, for assessing transfer efficiency onto pvdf, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or western blot detection reagents. Procedure from instruction manual for biorad transblot sd semidry electrophoretic transfer cell. Place your order before 8pm est for overnight delivery. Color prestained protein standard, broad range 10250 kda. M protein molecular mass marker pageruler unstained. Precision plus protein kaleidoscope prestained protein. Pdf bulletin 2998 download the protein standards application guide. Pageruler plus prestained protein ladders are a mixture of nine blue, orange and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and western blotting.
Thaw the ladder either at room temperature or at 3740c for a few minutes to dissolve precipitated solids. Prestained protein ladder, broad range 10230 kda is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis sdspage and western blotting. For more about pageruler prestained protein ladder concentration, please subscribe to our website newsletter now. Pageruler prestained protein ladder 2 x 250 l from thermo. Prestained protein ladder broad molecular weight 10245 kda.
Ladder is a prestained mixture of ten recombinant proteins ranging from 10 kda to 180 kda. The prestained protein marker triple color, edta free, 10250 kda is a prestained mixture of ten recombinant proteins ranging from 10 kda to 250 kda. Product information thermo scientific pageruler unstained. It is prestained, multicoloured peptides makes eassy to identify the protein of interst bands. Prestained protein ladder v is a threecolor protein standard with 10 prestained proteins covering a wide range of molecular weights for 10 to 180 kda. Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue, orange, and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and western blotting. Each marker is covalently bound to a pink or blue colour chromaphore to produce a ladder of evenly interspersed bands of uniform intensity. And to give sm6071 answer on the fermentas pageruler protein ladder sm ops question, i would agree that its then more specific fractionation techniques the fermfntas your workouts. Prestained protein ladder, broad range 10230 kda neb.
Pageruler prestained protein ladder pageruler plus prestained protein ladder prestained protein molecular weight marker unstained protein 816% trisglycine sdspage gel blot gel blot protein. The apparent molecular weight of the prestained proteins are given in the table below. In lowpercentage gels prestained protein ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10175 kda. Pageruler prestained protein ladder thermo fisher scientific. Unstained protein ladder is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10250 kda when analyzed by sdspage and stained with coomassie brilliant blue r250 1,2. Unstained versus prestained protein ladders scientist solutions. Blueray prestained protein marker, prestained protein mw. Precision plus protein prestained standards are available in dual color, all blue, kaleidoscope, and dual xtra options. M protein molecular mass marker pageruler unstained protein ladder fermentas. Pink plus prestained protein ladder 10175kda quantity.
Protein ladder, protein gel electrophoresis size marker, bluegreenred protein marker. Pageruler plus prestained protein ladder, 10 to 250 kda. The blueray prestained protein marker is a threecolor protein. Product information thermo scientific pageruler plus. Thermo scientific pageruler prestained protein ladder is a mixture of ten 10 blue, orange and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. Five microliters of benchmark prestained protein ladder was separated on a. M protein molecular mass marker pageruler unstained protein ladder fermentas from biology 402 at peking university.
Accuruler plus prestained protein ladder lamda biotech. Monitor protein migrationsample in sdspage, monitor protein transfer onto membranes during western blotting, sizing of proteins on sdspage and western blots introduction prestained protein ladder v is a threecolor protein standard with 10 prestained proteins covering a wide range of molecular weights for 10 to 180 kda. The protein ladder is conveniently packaged and ready to. Prestained protein standards life science research biorad. Shop online for a wide selection of thermo scientific pageruler plus prestained protein ladder, 10 to 250 kda mixture of 9 blue, orange and. The acuteband prestained protein ladder is a three color protein standard with 10. Briteruler prestained protein ladder 9306 biovision. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. Store the protein ladder for up to three months at 4c or for one year at 20c. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of western transfer efficiency on membranes and for sizing of proteins.
Prestained markers are brighter for visual robustness throughout western blotting to accurately estimate mw, monitor electrophoresis and transfer efficiency. The ladder consists of six bands which appear on the final western blot image. The benchmark prestained protein ladder is designed for use in sdspage using trisglycine gels for estimation of protein molecular weight, and evaluation of western transfer efficiency. Cst prestained protein marker, broad range 11190 kda. Applications of precision plus protein prestained standards. The protein ladder is supplied in a readytouse format for direct loading. The thermo scientific pageruler prestained protein.
The pageruler unstained broad range protein ladder is supplied readytouse. Prestained protein marker, broad range 11190 kda is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kda following electrophoresis. Benchmark prestained protein ladder allows you to accurately determine molecular weight and evaluate. Bluestain protein ladder, 11245 kda goldbio asset 1. In addition, the ladder proteins except for the 5kda peptide contain an integral streptag ii sequence and may be detected on western blots using streptactin conjugates. Prestained protein ladders can have a sligtly different mobility and mass than the same peotein ladder which is not prestained. Thermo scientific pageruler plus prestained protein. Colorplus prestained protein ladder, broad range 10230 kda. Page 4 benchmark prestained protein ladder limited use label. Mxstable prestained protein ladder dna protein ladders. Page 3 example the apparent molecular weights of the protein bands in benchmark prestained protein ladder are shown below. Acuteband prestained protein ladder save money for your lab. The thermo scientific pageruler prestained nir protein ladder is a mixture of 10proteins ranging from 11kda to 250kda, which produces 10 welldefined blue bands by sdspage see website for product images.
Under suggested conditions, blultra prestained protein ladder resolves 10 major bands in 412% bistris gelmes buffer and after western blotting to the nitrocellulose membrane. Proteins are covalently coupled with a blue chromophore except for two reference bands, one green at 25 kda and one red one at 75 kda respectively, when separated on sdspage trisglycine buffer. The apparent molecular weights of the colorplus prestained protein ladder bands were determined on invitrogen novex 1020% trisglycine sdspage gels 1. Proteins are used in routine laboratory procedures such as binding enzymes or coupling peptides to carrier proteins. Benchmark prestained protein ladder must show 10 major bands when separated by electrophoresis on a novex 420% trisglycine gel. Mixes very good with about why the right fermentas pageruler protein ladder sm eating plan works list in its category for more than a year. Prestained protein ladder is designed for monitoring protein separation during sdspolyacrylamide gel electrophoresis, verification of western transfer efficiency on membranes pvdf, nylon or nitrocellulose and for approximating the size of proteins. It is prestained and has multicoloured peptides which makes it easy to identify. Thermo scientific pageruler prestained protein ladders the thermo scientific pageruler and pageruler plus prestained protein ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein.
Opal prestained protein standard 10180kda is designed for monitoring protein separated during sdspolyacrylamide gel electrophoresis, verification of western blot transfer efficiency on membranes pvdf, nylon, or nitrocellulose and for approximate sizing of proteins. Does pierce pageruler prestained protein ladder a mix of. The protein concentrations are carefully balanced for even intensity. The molecular weights of the proteins have a lottolot variation of approximately 5%. Unstained protein ladder, broad range 10250 kda neb. Thermo scientific pageruler plus prestained protein ladder. Features of the benchmark prestained protein ladder. The accuruler plus prestained protein ladder contains 12 prestained proteins, covering a wide range of molecular weights from 10 to 245kda. Pageruler prestained protein ladder, 10 to 180kda sdspage. Coloured reference bands serve as visual indicators of electrophoresis run progression and the efficiency of western transfer onto membranes. Instructions pageruler prestained nir protein ladder. Mix thoroughly to ensure the solution is homogeneous.
Product information thermo scientific pageruler unstained protein. Pageruler prestained protein ladder, 10 to 180 kda. We checked neural protein expression using this protein ladder as a marker for proteins of various molecular weights. Opal prestained protein standard 10180kda mb2090500. Biotinylated protein ladder detection pack is designed to detect the molecular weight ladders on western blots when using the horseradish peroxidase hrp based western detection system. The blue prestained protein standard, broad range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed. Benchmark prestained protein ladder thermo fisher scientific.
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